This was something of a trick question, the reference to the Tudors leading most people (at least in the quiz I took part in) down the path of answering "Henry the Eighth."
When you heard the answer, the clue was there in the date: 1521.
Wikipedia notes that it was in 1532, after Parliament recognised the King's status as head of the church in England and abolished the right of appeal to Rome, that "Pope Clement [VII] took the step of excommunicating Henry [VIII] and Thomas Cranmer, although the excommunication was not made official until some time later." It adds, in a footnote, that "On 11 July 1533 Pope Clement VII 'pronounced sentence against the King, declaring him excommunicated unless he put away the woman he had taken to wife, and took back his Queen during the whole of October next.' Clement died on 25 September 1534. On 30 August 1535 the new pope, Paul III, drew up a bull of excommunication which began 'Eius qui immobilis'. G. R. Elton puts the date the bull was made official as November 1538. On 17 December 1538 Pope Paul III issued a further bull which began 'Cum redemptor noster', renewing the execution of the bull of 30 August 1535, which had been suspended in hope of his amendment."
In other words (if I understand this correctly): the excommunication of Henry VIII was threatened in 1532, but wasn't completed until 1538. It spanned the reigns of two popes: Clement VII and Paul III.
The woman referred to by Pope Clement as the one Henry had "taken to wife" was Anne Boleyn, and "his Queen" was Catherine of Aragon.
© Macclesfield Quiz League 2018